The CME modified by azure I, a new electron-transfer mediator, which w
as electrodeposited onto the surface of spectrographic graphite electr
ode, shows significantly electrocatalytic activity to the reduction of
hemoglobin. In the potential range from 0.3 V to -0.7 V (vs. SCE), th
e mediator azure I behaves as one-electron reversible redox process in
volving two protons in acid solution and one proton in basic solution.
In the medium of 0.2 mol/L HAc-NaAc(pH = 5.5). The formal potential E
(0') of the electrodeposited azure 1 is -0.21 V and the apparent elect
ron-transfer rate constant is 0.69 s(-1). This CME shows a good stabil
ity.