CHEMILUMINESCENCE DETERMINATION OF D-AMIN O-ACID BASED ON IMMOBILIZEDENZYMES

D-amino acid oxidase and HRP were separately immobilized on chitosan a dsorbed on the silica gels to develop a chemiluminescent method, which was combined with two oxidation reactions of D-amino acid producing H 2O2 catalyzed by D-amino acid oxidase and of Luminol-H2O2 generating C L catalyzed by HRP together, also was stopped-flow technique used in t his system. It has a good selectivity and sensitivity. Under optimized conditions, the detection limit is 1.8 x 10(-8) g/mL, the liner range of D-amino acid concentration was from 8 x 10(-8) to 1.4 x 10(-6) g/m L. It was practicable to determine D-amino acid in serum directly with satisfactory results.