TEST-YOLK MEDIA AND SPERM QUALITY

Media prepared with egg yolk and two buffers, TES and Tris, called TES T-yolk have been shown to have beneficial effects on the survivability , fertilizing capacity and storage potential of human spermatozoa, Egg yolk lipoproteins are the critical compounds for the beneficial effec ts,,vith a synergistic effect due to the TES-Tris buffer component, TE ST-yolk media have been used in the sperm penetration assay, the hemiz ona assay, sperm preparation for clinical in-vitro fertilization, arti ficial insemination with homologous spermatozoa, cryopreservation, spe rm samples with a positive antisperm antibody test, and preparation fo r techniques requiring capacitated spermatozoa. Few harmful consequenc es of TEST-yolk have been reported, although controlled trials are req uired to evaluate therapeutic effects in the treatment of male inferti lity.