AN ELECTROSPRAY INVESTIGATION ON IN-VITRO GLYCATION OF RIBONUCLEASE

The in vitro reactions of RNase with different concentrations of gluco se or fructose have been studied by means of electrospray mass spectro metry coupled with microcolumn high performance liquid chromatography. The results obtained have shown that, subsequent to the protein glyca tion, a series of cross-linking products are generated. Their molecula r weights demonstrate that severe degradation processes of the proteic substrate takes place after the cross-linking process.