STEROID SEX-HORMONES AND MACROPHAGE FUNCTION - REGULATION OF CHEMILUMINESCENCE AND PHAGOCYTOSIS

PROBLEM: Female sex hormones modulate a variety of humoral and cell-me diated immunologic functions. In this study, the effects of estrogen, progesterone, and testosterone on the chemiluminescence (CL) response and phagocytic ability of male rat peritoneal macrophages (M phi) were examined. METHOD: In M phi pretreated with 10(-2) ng/ml of 17 beta-es tradiol (E(2)) for 20 hours, the CL generated in response to phorbol m yristate acetate (PMA), 1,2-dioctanoyl-rac-glycerol (C8:0), or opsoniz ed zymosan (OZ) was significantly increased by 135%, 140%, and 136% of control values, respectively. In addition, M phi treated with 10(-5) ng/ml or 10 ng/ml of E(2) exhibited a significantly greater PMA-or OZ- stimulated CL response than did untreated controls. RESULTS: At 10(-2) ng/ml, progesterone enhanced and testosterone reduced the CL response , but these changes were not statistically significant. In time course studies, the PMA-stimulated CL response of M phi treated with 10(-2) ng/ml of E(2) or progesterone for 5 h was significantly less than that of the untreated group. In the presence of endotoxin (12 pg/ml), the CL response in M phi treated with E(2) or testosterone was significant ly depressed as compared to untreated controls. Phagocytosis of opsoni zed sheep erythrocytes also was significantly enhanced (140% to 190% o f control) when M phi were pretreated with 10(-12) M to 10(-8) M of ei ther E(2) or progesterone. CONCLUSIONS: These findings suggest that, a t physiological concentrations, E(2) is capable of modulating both CL generation and phagocytic uptake by M phi in a manner not shared by ot her steroid hormones.