SIMPLIFIED RIBOPROBE PURIFICATION USING TRANSLUCENT STRAWS AS GEL TUBES

Authors
KOL S BENSHLOMO I ADASHI EY ROHAN RM
Citation
S. Kol et al., SIMPLIFIED RIBOPROBE PURIFICATION USING TRANSLUCENT STRAWS AS GEL TUBES, GENET A-BIO, 12(3-4), 1996, pp. 129-132
Citations number
1
Categorie Soggetti
Genetics & Heredity","Biochemical Research Methods
Journal title
GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING → ACNP
ISSN journal
10503862
Volume
12
Issue
3-4
Year of publication
1996
Pages
129 - 132
Database
ISI
SICI code
1050-3862(1996)12:3-4<129:SRPUTS>2.0.ZU;2-C
Abstract
Gel purification of radioactive riboprobes enhances the quality of the ribonuclease protection assay. A simple and effective method for ribo probe purification is described. The method uses acrylamide gels in pl astic tubes to achieve electrophoretic separation of the RNA polymeras e products.