CONSTRUCTION AND UTILITY OF A HUMAN-CHROMOSOME 22-SPECIFIC FOSMID LIBRARY

We have previously demonstrated the capability of the Fosmid vector ba sed on Escherichia coli F-Factor replicon to stably propagate cosmid-s ized human genomic DNA fragments. Using the Fosmid vector, we have con structed and arrayed a 10x human chromosome 22-specific library, partl y by picking human positive clones from a total Fosmid library constru cted using DNA from human-hamster hybrid cell line containing human ch romosome 22, and partly by using flow-sorted chromosomal DNA. The clon es and physical contig maps of the clones in the library will serve as a valuable resource for detailed analysis of the chromosome by somal regions of interest spanning several hundred kilobases to a megabase, it is necessary to rapidly identify subsets of the Fosmid clones from the library that cover such regions. In this report, we describe a met hod of using random amplification products derived from YAC clones to rapidly identify a subset of Fosmid clones that cover a specific genom ic subregion.