T-VECTOR CLONING AND HIGH-PERFORMANCE PCR WITH SUPERTTH FROM THERMUS-THERMOPHILUS

Authors
LEAL JFM LOPEZBAREA J DORADO G
Citation
Jfm. Leal et al., T-VECTOR CLONING AND HIGH-PERFORMANCE PCR WITH SUPERTTH FROM THERMUS-THERMOPHILUS, GENET A-BIO, 12(2), 1995, pp. 119-121
Citations number
18
Categorie Soggetti
Genetics & Heredity","Biochemical Research Methods
Journal title
GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING → ACNP
ISSN journal
10503862
Volume
12
Issue
2
Year of publication
1995
Pages
119 - 121
Database
ISI
SICI code
1050-3862(1995)12:2<119:TCAHPW>2.0.ZU;2-#
Abstract
The SuperTth DNA polymerase from Thermus thermophilus exhibits templat e-independent terminal transferase (extendase) activity. This enzyme i s proposed as a cheap alternative for both high performance PCR as wel l as quick T-vector cloning of amplicons, including reverse transcript ion and cDNA cloning.