Je. Purcell et Pav. Anderson, ELECTRICAL RESPONSES TO WATER-SOLUBLE COMPONENTS OF FISH MUCUS RECORDED FROM THE CNIDOCYTES OF A FISH PREDATOR, PHYSALIA-PHYSALIS, Marine and freshwater behaviour and physiology, 26(2-4), 1995, pp. 149-162
Physalia physalis, the Portuguese man of war, consumes mostly fish and
fish larvae. Intracellular recordings from nematocyst-containing cell
s (cnidocytes) in small pieces of Physalia tentacle were used to quant
ify the electrical responses to diluted and filtered fish skin mucus,
1-100 x 10(-6) M amino acids, monosaccharides, and nucleosides, and se
awater, which were delivered upstream of the tissue. Seawater caused r
esponses (one pulse only) in about 10% of the applications. Fish mucus
extract elicited responses in all applications, producing 1-18 depola
rizing pulses (20 mV maximum amplitude). The pulses were characteristi
c of post-synaptic potentials (EPSPs). Lucifer yellow and biocytin dye
injections showed that the cnidocytes were not electrically coupled.
Simultaneous records from two cnidocytes following mucus applications
were identical. We propose, therefore, that the chemoreceptors are not
on the cnidocytes, but are probably on sensory neurons that innervate
clusters of cnidocytes. A < 3,000 MW fraction of mucus elicited respo
nses indistinguishable from whole mucus extract. Higher molecular weig
ht fractions caused no response. The various test solutions had lower
percentages of response (47-92%) and produced significantly fewer puls
es than the mucus extract. We conclude that prey capture in Physalia i
s facilitated by chemicals present in the mucus covering their fish pr
ey. The chemical stimuli probably sensitize the nematocysts to dischar
ge upon mechanical stimulation.