GENETIC-ANALYSIS AND NOMENCLATURE FOR 7 ISOZYME SYSTEMS IN BRASSICA-NIGRA, BRASSICA-OLERACEA AND BRASSICA-CAMPESTRIS

Citation
Am. Chevre et al., GENETIC-ANALYSIS AND NOMENCLATURE FOR 7 ISOZYME SYSTEMS IN BRASSICA-NIGRA, BRASSICA-OLERACEA AND BRASSICA-CAMPESTRIS, Plant breeding, 114(6), 1995, pp. 473-480
Citations number
41
Categorie Soggetti
Plant Sciences",Agriculture
Journal title
ISSN journal
01799541
Volume
114
Issue
6
Year of publication
1995
Pages
473 - 480
Database
ISI
SICI code
0179-9541(1995)114:6<473:GANF7I>2.0.ZU;2-3
Abstract
General criteria for the assignment of names to enzyme systems, region s of activity, isozyme loci and allozymes have been lacking in crucife r species. This paper proposes a standard nomenclature for seven isozy me systems in the three diploid species of U's triangle: Brassica nigr a, B. oleracea and B. campestris. Gel/electrode buffers, which provide d the best resolution for seven isozyme systems, acid phosphatase (APS ), aconitase (AGO), leucine aminopeptidase (LAP), 6-phosphogluconate d ehydrogenase (6 PGD), phosphoglucoisomerase (PGI), phosphoglucomutase (PGM), and triosephosphate isomerase (TPI), were proposed as standards . Isozyme genetic analysis was determined for B. oleracea and B. campe stris from previous studies and by segregation of selfed progenies of heterozygous B. nigra plants. Several populations were studied and 148 allozymes at the 18 loci observed were described for the three specie s. Their relative mobility was studied using a pure line of oilseed ra pe as reference. The comparison of the different alleles within and be tween the species is discussed.