Am. Chevre et al., GENETIC-ANALYSIS AND NOMENCLATURE FOR 7 ISOZYME SYSTEMS IN BRASSICA-NIGRA, BRASSICA-OLERACEA AND BRASSICA-CAMPESTRIS, Plant breeding, 114(6), 1995, pp. 473-480
General criteria for the assignment of names to enzyme systems, region
s of activity, isozyme loci and allozymes have been lacking in crucife
r species. This paper proposes a standard nomenclature for seven isozy
me systems in the three diploid species of U's triangle: Brassica nigr
a, B. oleracea and B. campestris. Gel/electrode buffers, which provide
d the best resolution for seven isozyme systems, acid phosphatase (APS
), aconitase (AGO), leucine aminopeptidase (LAP), 6-phosphogluconate d
ehydrogenase (6 PGD), phosphoglucoisomerase (PGI), phosphoglucomutase
(PGM), and triosephosphate isomerase (TPI), were proposed as standards
. Isozyme genetic analysis was determined for B. oleracea and B. campe
stris from previous studies and by segregation of selfed progenies of
heterozygous B. nigra plants. Several populations were studied and 148
allozymes at the 18 loci observed were described for the three specie
s. Their relative mobility was studied using a pure line of oilseed ra
pe as reference. The comparison of the different alleles within and be
tween the species is discussed.