GAIN OR LOSS OF SINGLE CHROMOSOMES IN WHEAT RYE ADDITION LINES AND IN6X TRITICALE DETECTED BY FLOW-CYTOMETRY

Flow cytometric DNA analysis was used to study changes in nuclear DNA content induced by the gain or loss of chromosomes or chromosome arms in wheat-rye addition lines and in triticale. The amount of DNA presen t was determined by comparison with internal reference values obtained from euploid plants. The smallest difference in the DNA level which c ould be detected between aneuploid and euploid plants was 1.84%. Thus, it was possible to determine aneuploidy in all cases, except when ver y short telocentric rye chromosomes were added to wheat (addition line IRS). The study has proved that how cytometry is an efficient and sim ple way to discriminate between euploid and aneuploid plants in wheat and triticale.