Rg. Roussev et al., DEVELOPMENT AND VALIDATION OF AN ASSAY FOR MEASURING PREIMPLANTATION FACTOR (PIF) OF EMBRYONAL ORIGIN, American journal of reproductive immunology [1989], 35(3), 1996, pp. 281-287
PROBLEM: Tests to determine presence of embryos prior to implantation
are needed. METHODS: Sera from women after embryo transfer were tested
for preimplantation factor (PIF) using the lymphocyte/platelet bindin
g assay. Autorosettes were counted using blood type O+ donor lymphocyt
es and platelets incubated with blinded serum in the presence of antiC
D2 antibody and rabbit complement. Human chorion gonadotropin (hCG) co
ncentrations were determined 7 days later and compared with results of
the lymphocyte/platelet assay. Implantation was confirmed by ultrason
ographic evidence of presence of an intrauterine gestational sac. The
roles of platelet activating factor (PAF) and chaperonin 10 in the obs
erved phenomena were studied experimentally. RESULTS: Significantly mo
re lymphocyte/platelet rosette formations were observed when sera from
women who successfully implanted were compared to sera from women who
failed to implant. Neither PAF nor chaparonin added to the tested ser
a controls influenced the percentage of lymphocyte/platelets rosettes.
CONCLUSIONS: PIF is a likely candidate to be the next frontier of dia
gnosing the presence of viable preimplantation embryos in vivo.