SPECTROFLUOROMETRIC DETERMINATION OF GALLIUM WITH N-(3-HYDROXY-2-PYRIDYL)SALICYLALDIMINE AND ITS APPLICATION TO THE ANALYSIS OF BIOLOGICAL SAMPLES

The fluorimetric determination of gallium at the nanogram level, based on the formation of a fluorescent complex between Ga(III) and N-(3-hy droxy-2-pyridyl)salicylaldimine (3-OH-PSA), is proposed. With excitati on at 397 nm, the chelate has a maximum emission at 498 nm. The reacti on is carried out at acidic pH in aqueous-DMF medium (40% v/v DMF). Th e influence of the reaction variables are discussed. The detection lim it is 0.9 ng ml(-1) and the range of application is 1-125 ng ml(-1). T he relative error of the method is +/- 1.6%. The proposed method has b een applied to the determination of gallium in biological samples.