PHOSPHOLIPID-COMPOSITION OF ERYTHROCYTE-MEMBRANES AND PLASMA OF MAMMALIAN BLOOD INCLUDING AUSTRALIAN MARSUPIALS - QUANTITATIVE P-31 NMR ANALYSIS USING DETERGENT
Mh. Nourisorkhabi et al., PHOSPHOLIPID-COMPOSITION OF ERYTHROCYTE-MEMBRANES AND PLASMA OF MAMMALIAN BLOOD INCLUDING AUSTRALIAN MARSUPIALS - QUANTITATIVE P-31 NMR ANALYSIS USING DETERGENT, Comparative biochemistry and physiology. B. Comparative biochemistry, 113(2), 1996, pp. 221-227
The phospholipid classes of erythrocyte membranes and plasma from seve
ral domestic animals and marsupials were quantified by P-31 NMR using
detergents. Washed erythrocyte samples were thoroughly haemolysed by t
ip-sonication and dissolved in sodium cholate; plasma samples were dis
solved in Triton X-100. The species studied were: common wombat (Vomba
tus ursinus), black-striped wallaby (Macropus dorsalis), bandicoot (Is
oodon macrocarpus), Eastern grey kangaroo (Macropus giganteus), Tammar
wallaby (Macropus eugenii), sheep (Ovis aries), goat (Capra hircus),
cattle (Bos taurus), horse (Equus caballus), dog (Canus familiaris) an
d rabbit (Orytolagus caniculus). There were considerable species varia
tions in the relative abundance oi erthrocyte and plasma phospholipid
classes. The variations may be attributed to the habitats and diets of
the animals as well as to their phylogenetic differences.