ACCUMULATION OF GOSSYPOL ENANTIOMERS IN OVINE TISSUES

Tissue residue levels of gossypol enantiomers in cottonseed-fed and le thally intoxicated lambs were determined by the high-performance liqui d chromatography-ultraviolet detection method. Gossypol was derivatize d with (+)-2-amino-1-propanol and separated with a reversed-phase C18 column and the elution of analytes was monitored at 254 nm. The highes t residue level was found in the liver tissue (318-416 ng total gossyp ol/mg dry tissue), and the residue of (-)-gossypol was higher than (+) -gossypol in the heart, muscle and spleen. The detection limit was 2 n g, and the detector response of gossypol-amine adducts was linear betw een 2 and 100 ng enantiomers.