DETERMINATION OF THE ALKALOIDS IN EVODIAE FRUCTUS BY HIGH-PERFORMANCELIQUID-CHROMATOGRAPHY

A high-performance liquid chromatographic method for the simultaneous determination of four indolequinazoline alkaloids (carboxyevodiamine, dehydroevodiamine, evodiamine and rutaecarpine) and eight quinolone al kaloids (1-methyl-2-nonyl-4 (1H)-quinolone, 1-methyl-2-[(Z)-6-undeceny l]-4(1H)-quinolone, 1-methyl-2-undecyl-4(1H)-quinolone, evocarpine, l- 2-[(6Z,9Z)-6,9-pentadecadienyl]-4(1H)-quinolone, dihydroevocarpine, 1- methyl-2-[(Z)-10-pentadecenyl]-4(1H)-quinolone and 1-methyl-2-[(Z)-6-p entadecenyl]-4(1H)-quinolone) in Evodiae Fructus was developed. The me thod was carried out by using a Capcell C-18 SG-120 column with a grad ient solvent system of acetate buffer-acetonitrile-methanol and a UV d etector (250 nm), and the contents of the alkaloids in a non-pretreate d Evodiae Fructus extract could easily be determined within 40 min. Va lidation of this method is described.