ANTIGEN RETRIEVAL, SIGNAL AMPLIFICATION AND INTENSIFICATION IN IMMUNOHISTOCHEMISTRY

Authors
WERNER M VONWASIELEWSKI R KOMMINOTH P
Citation
M. Werner et al., ANTIGEN RETRIEVAL, SIGNAL AMPLIFICATION AND INTENSIFICATION IN IMMUNOHISTOCHEMISTRY, HISTOCHEM C, 105(4), 1996, pp. 253-260
Citations number
55
Categorie Soggetti
Cell Biology",Microscopy
Journal title
HISTOCHEMISTRY AND CELL BIOLOGY
ISSN journal
09486143 → ACNP
Volume
105
Issue
4
Year of publication
1996
Pages
253 - 260
Database
ISI
SICI code
0948-6143(1996)105:4<253:ARSAAI>2.0.ZU;2-O
Abstract
In this overview we emphasize new methods of improving immunohistochem ical results in formaldehyde-fixed tissue samples. The benefit of heat -induced antigen retrieval in demasking of concealed epitopes is demon strated. We provide guidance on the influence of heat-induced antigen retrieval in commonly applied monoclonal and polyconal antibodies. Mor eover, we show the promising methods of signal amplification using bio tinylated tyramine and signal intensification of diaminobenzidine reac tion products by metallic ions.