FLASH CHIRAL CHROMATOGRAPHY WITH CELLULOSE TRIS(3,5-DIMETHYLPHENYLCARBAMATE)-COATED PHASES IMPROVED RESOLUTION OF BASIC ANALYTES

The preparative resolution of some chiral basic analytes on a flash ch iral chromatography column packed with a cellulose carbamate-coated ph ase is described. Compared with separations obtained, on a column pack ed with 20% (w/w) cellulose tris(3,5-dimethylphenylcarbamate) coated o nto underivatised flash silica (40-63 mu m Davisil, 150 Angstrom), a s ubstantial improvement was observed when the support medium was change d to octadecylated silica (37-55 mu m Bondapak ODS, 300 Angstrom). The ODS phase is extremely effective for the preparative separation of ba sic analytes such as beta-blockers and the optimum conditions can be r eadily established by the use of a related high-pressure liquid chroma tography column containing the chiral carbamate coated onto Nucleosil- ODS (5 mu m, 300 Angstrom).