INFLUENCE OF THE KIND OF THE ALCOHOLIC MODIFIER ON CHIRAL SEPARATION ON A CHIRALPAK AD COLUMN

The enantioselective separation of substituted 3-phenoxy-1,2-propanedi ols (PPDs) and aziridinecarboxylic acid derivatives (ACAs) on Chiralpa k AD, an amylose-based chiral stationary phase, was investigated. All solutes could be separated using hexane-alcohol mobile phases. The ret ention times for sixteen out of seventeen substituted PPDs and from al l the ACAs increased on changing the alcoholic modifier from 2-propano l to ethanol or ethanol-methanol mixtures. The separation factors of s ixteen out of seventeen PPDs and nine out of twelve ACAs were improved using ethanol or ethanol-methanol mixtures instead of 2-propanol.