HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH NONAQUEOUS SOLVENTS - RELATIVE ELUTION STRENGTH ON A POLYSTYRENE-DIVINYLBENZENE COLUMN

A polymeric resin-based stationary phase was used to study the retenti on of alkylphenols, alkylbenzenes, substituted benzenes and fused-ring compounds with different eluents. Various 100% organic solvents were used as eluents in order to observe the relative ability to solvents t o elute compounds of given chemical structure, Plots of log k' vs. num ber of carbons in a chain attached to the aromatic ring showed linear correlations with slopes that are dependent on solvent type and solute functionality. Of the solvents studied, acetonitrile exhibited the hi ghest solvating ability for the benzene ring and for several functiona l groups. Ethanol was shown to have the strongest eluting power for th e methylene group. The relative ability of several solvents to elute p olynuclear aromatic hydrocarbons in solid-phase extraction was determi ned. The functional group contribution (tau) was calculated for select ed compounds and acceptable agreement was found in the correlation bet ween experimental and predicted values for solute capacity factor.