HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF PENICILLINS BY MEANS OF AUTOMATED SOLID-PHASE EXTRACTION AND PHOTOCHEMICAL DEGRADATION WITH ELECTROCHEMICAL DETECTION
S. Lihl et al., HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF PENICILLINS BY MEANS OF AUTOMATED SOLID-PHASE EXTRACTION AND PHOTOCHEMICAL DEGRADATION WITH ELECTROCHEMICAL DETECTION, Journal of chromatography, 729(1-2), 1996, pp. 229-235
Citations number
25
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
An automated precolumn exchange system for on-line solid-phase extract
ion (OPS-2) coupled to liquid chromatography with photochemical degrad
ation and electrochemical detection was used for the determination of
residual amounts of penicillin G, penicillin V, oxacillin, cloxacillin
and dicloxacillin. A 5-10-fold increase in sensitivity was obtained w
hen compared with direct UV detection of penicillins at 225 nm. The sy
stem is recommended for samples that have received an immunoaffinity c
lean-up. Analysis of bovine muscle tissue indicated, however, that thi
s procedure could also be suitable for the determination of penicillin
G at its maximum residue limit of 50 mu g/kg even with conventional s
olvent partitioning for the first clean-up steps.