DETERMINATION OF FCE-26644, A NEW POLYSULPHONATED DERIVATIVE OF DISTAMYCIN-A, IN MONKEY PLASMA BY REVERSED-PHASE ION-PAIR HIGHPERFORMANCE LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION

A sensitive and selective ion-pair high-performance liquid chromatogra phic method for the determination of ole)carbonylimino]}-bis(1,3-napht halenedisulphonic acid), tetrasodium salt in monkey plasma has been de veloped. The compound and internal standard (bromphenol blue) were ext racted from plasma samples with methylene chloride (twice) after depro teination with acetonitrile and addition of the ion-pairing agent (tet rabutylammonium hydroxide). The combined organic phases were dried, th e residue dissolved in the mobile phase and then analysed by reversed- phase ion-pair liquid chromatography under isocratic conditions. The H PLC analysis time was about 20 min. Quantitation was achieved by UV de tection at 323 nm. The linearity, precision and accuracy of the method were evaluated. The limit of quantitation was 0.3 mu g/ml plasma. No interference from blank monkey, mouse, rat, dog and human plasma was o bserved. The suitability of the method for in vivo samples was checked by analysis of plasma samples drawn from three male cynomolgus monkey s that had received a 20 mg/kg single i.v. dose of the test compound.