R. Metz et al., IMPROVED DETERMINATION OF SULFADIAZINE IN HUMAN PLASMA AND URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography, 729(1-2), 1996, pp. 243-249
Citations number
8
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A high-performance liquid chromatographic method for the determination
of sulfadiazine in human plasma and human urine was developed and val
idated. The method involves the acid extraction of drug and internal s
tandard from plasma with ethyl acetate followed by evaporation and rec
onstitution in mobile phase. Urine samples were simply diluted with pu
rified water. Recovery, linearity, intra- and inter-day variation of s
ulfadiazine were tested and found appropriate. The quantitation range
was 0.0299-15.2 mu g/ml for plasma samples and 0.578-148.8 mu g/ml for
urine samples. The method is suitable for the quantitation of sulfadi
azine from pharmacokinetic studies.