P. Muth et al., SENSITIVE DETERMINATION OF NITROFURANTOIN IN HUMAN PLASMA AND URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography, 729(1-2), 1996, pp. 251-258
Citations number
6
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A highly sensitive and selective HPLC method was developed and validat
ed for the determination of nitrofurantoin in human plasma and urine.
The method involves the liquid-liquid extraction of drug and internal
standard from plasma with ethyl acetate followed by evaporation and re
constitution in mobile phase. Urine samples were simply diluted with p
urified water. UV detection was done at 370 nm. The limit of quantific
ation for nitrofurantoin in plasma was 0.010 mu g/ml. In urine nitrofu
rantoin could be quantified down to 0.380 mu g/ml. Linearity was prove
n over the whole calibration range in plasma (2.48-0.0100 mu g/ml) as
well as in urine (187 mu g/ml-0.380 mu g/ml). The method was validated
according to Good Laboratory Practice guidelines and its suitability
was demonstrated by analysis of samples from a pharmacokinetic study.