FLUOROMETRIC METHOD OF ANALYSIS FOR D-NORPSEUDOEPHEDRINE HYDROCHLORIDE, GLYCINE AND L-GLUTAMIC ACID BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

The determination of D-norpseudoephedrine HCl, an appetite suppressant , and glycine and L-glutamic acid, both dietary supplements, in pharma ceutical formulations and dissolution media using reversed-phase high- performance liquid chromatography (HPLC) combined with fluorimetric de tection is reported. A reagent solution containing o-phthalaldehyde an d a reducing agent, mercaptoethanol, appeared to be the most favourabl e reagent for derivatising the three compounds. The use of this HPLC m ethod allowed for selective and quantitatively accurate analysis and w as sufficiently specific, precise and sensitive for analytical charact erisation.