K. Akasaka et al., FLUOROMETRIC-DETERMINATION OF DIARRHETIC SHELLFISH TOXINS IN SCALLOPSAND MUSSELS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography, 729(1-2), 1996, pp. 381-386
Citations number
15
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
The fluorimetric determination of okadaic acid (OA) and dinophysistoxi
n-1 (DTX-1), the principal toxins of diarrhetic poisoning, is reported
. The digestive glands of mussels or scallops were homogenized with 2-
propanol. OA and DTX-1 were extracted from the homogenate, with hexane
-ethyl acetate and labelled with 2,3-(anthracenedicarboximido)ethyl tr
ifluoromethanesulfonate in dry acetonitrile. After cleaning up by pass
age through a short silica gel column, the fluorescent derivatives wer
e determined by HPLC. The derivatives were at first separated on a Dev
elosil Ph-5 column, and only the target fraction obtained was introduc
ed into a Develosil ODS K-5 column by a valve-switching device. Both t
oxins were determined in the range 2.5-500 pg, and the detection limit
s were 0.8 pg (OA) and 1.3 pg (DTX-1) with a signal-to-noise ratio of
3.