AUTOMATIC HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF DIARRHETIC SHELLFISH POISON

Authors
HUMMERT C SHEN JL LUCKAS B
Citation
C. Hummert et al., AUTOMATIC HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF DIARRHETIC SHELLFISH POISON, Journal of chromatography, 729(1-2), 1996, pp. 387-392
Citations number
13
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography → ACNP
Volume
729
Issue
1-2
Year of publication
1996
Pages
387 - 392
Database
ISI
SICI code
Abstract
A rapid HPLC method with fluorescence detection for the determination of okadaic acid (OA) and dinophysistoxin-1 (DTX-1) in mussels and muss el products is presented. For fluorescence labeling of OA and DTX-1, 9 -anthryldiazomethane (ADAM) is used. HPLC with a column-switching syst em is proposed to avoid time-consuming clean-up procedures after deriv atization of sample extracts with ADAM. The column-switching system as well as the chromatographic conditions and detection are described.