ANALYSIS OF BITTER ESSENTIAL OILS FROM ORANGE AND GRAPEFRUIT BY HIGHPERFORMANCE LIQUID-CHROMATOGRAPHY WITH MICROBORE COLUMNS

The analysis of bitter orange and grapefruit essential oils (non-volat ile fraction) was carried out by HPLC in normal- and reversed-phase mo de with UV detection. These oils were compared with the sweet orange a nd mandarin essential oils, analyzed previously. For the identificatio n of chromatographic peaks, fractionation by RP-HPLC was carried out. The purified fractions were analyzed by GC-MS and LC-MS. Some new comp ounds were found, together with many others already identified in diff erent citrus essential oils.