SOLID-PHASE EXTRACTION AND REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF FREE PHENOLIC-ACIDS IN SOME ECHINACEA SPECIES

A new method is described combining solid-phase extraction (SPE) and r eversed-phase high-performance liquid chromatography (RP-HPLC) for the isolation, purification as well as qualitative and quantitative deter mination of free phenolic acids in six Echinacea species. Plant extrac ts were purified and phenolic acids isolated on octadecyl and quaterna ry amine Bakerbond SPE columns; final eluates were analysed by RP-HPLC . Significant differences in the composition and amount of phenolic ac ids within Echinacea genus have been shown. The method can be used for quick screening analysis of the content of phenolic acids in plant ma terial.