SIMULTANEOUS HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OFVISOLTRICIN, ACUMINATOPYRONE AND CHLAMYDOSPOROLS IN FUSARIUM CULTURESON MAIZE

Visoltricin (VIS), acuminatopyrone (ACP), clamydosporol (CL), isochlam ydosporol (ICL) and chlamydospordiol (DIOL), recently characterized Fu sarium metabolites, were separated on a polymeric RP-18 column eluted with acetonitrile-0.01% ammonia solution (35:65) at 1 ml/min and detec ted with a diode-array UV detector. The presence of ammonia in the mob ile phase improved the shape of the CL and VIS peaks. The use of a pol ymeric column was required owing to the basic pH of the mobile phase. Maize cultures of several strains of F. tricinctum and F. chlamydospor um were analysed with this procedure after extraction with aqueous met hanol, partitioning with methylene chloride and clean-up with a C-18 m inicolumn. VIS was produced only by F. tricinctum, whereas ACP and chl amydosporols were produced by both Fusarium species.