M. Solfrizzo et A. Visconti, SIMULTANEOUS HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OFVISOLTRICIN, ACUMINATOPYRONE AND CHLAMYDOSPOROLS IN FUSARIUM CULTURESON MAIZE, Journal of chromatography, 730(1-2), 1996, pp. 69-73
Citations number
12
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Visoltricin (VIS), acuminatopyrone (ACP), clamydosporol (CL), isochlam
ydosporol (ICL) and chlamydospordiol (DIOL), recently characterized Fu
sarium metabolites, were separated on a polymeric RP-18 column eluted
with acetonitrile-0.01% ammonia solution (35:65) at 1 ml/min and detec
ted with a diode-array UV detector. The presence of ammonia in the mob
ile phase improved the shape of the CL and VIS peaks. The use of a pol
ymeric column was required owing to the basic pH of the mobile phase.
Maize cultures of several strains of F. tricinctum and F. chlamydospor
um were analysed with this procedure after extraction with aqueous met
hanol, partitioning with methylene chloride and clean-up with a C-18 m
inicolumn. VIS was produced only by F. tricinctum, whereas ACP and chl
amydosporols were produced by both Fusarium species.