Cn. Carducci et al., APPLICATION OF EXTRACTION DISKS IN DISSOLUTION TESTS OF CLENBUTEROL AND LEVOTHYROXINE TABLETS BY CAPILLARY ELECTROPHORESIS, Journal of chromatography, 730(1-2), 1996, pp. 313-319
Citations number
17
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Sample preparation procedures using octadecyl (C-18) extraction disks
were developed to obtain accurate and reproducible results for determi
nations of clenbuterol (20 mu g per dose) and levothyroxine (100 mu g
per dose) in dissolution media of solid oral dosage forms. Preconcentr
ation of samples allowed final concentrations of 1.1 mu g/ml of clenbu
terol and 4.0 mu g/ml of levothyroxine to be reached prior to CE analy
sis. The results obtained by CE were in good agreement with those of H
PLC. The precision of the migration time, peak area, peak height and a
ccuracy were determined in both intra-day (n = 6) and inter-day (n = 1
8) assays. Linearity was demonstrated over the ranges 0.5-80.0 mu g/ml
of clenbuterol and 1.0-30.0 mu g/ml of levothyroxine. The mean recove
ries were higher than 94.0%, ranging from 50 to 125% levels with respe
ct to dose potencies. The proposed methodology may be generally applie
d to determine drugs at ng/ml concentrations.