DETERMINATION OF ALTERNARIOL AND ALTERNARIOL METHYL-ETHER IN APPLE JUICE USING SOLID-PHASE EXTRACTION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Authors
DELGADO T GOMEZCORDOVES C SCOTT PM
Citation
T. Delgado et al., DETERMINATION OF ALTERNARIOL AND ALTERNARIOL METHYL-ETHER IN APPLE JUICE USING SOLID-PHASE EXTRACTION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography, 731(1-2), 1996, pp. 109-114
Citations number
29
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography → ACNP
Volume
731
Issue
1-2
Year of publication
1996
Pages
109 - 114
Database
ISI
SICI code
Abstract
The present work describes a new method for determination of alternari ol (AGH) and alternariol methyl ether (AME) in apple juice using solid -phase extraction (SPE) columns for extraction and cleanup of samples for high-performance liquid chromatography (HPLC). Chromatograms of sp iked samples show that both toxins can be easily detected without inte rferences, and good recoveries for AOH (82.8 +/- 7.4%) and AME (91.9 /- 6.1%) with detection limits as low as 1.6 and 0.7 mu g/l, respectiv ely, were obtained.