HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF PAIRS OF ISOTOPIC LABELED (DEUTERIUM PROTIUM) MOLECULES/

The separation of n-octane-d(0) and n-octane-d(18) has been accomplish ed using a liquid chromatographic technique; baseline separation was e xhibited with the n-octane-d(18) eluting first. Thus, the liquid chrom atographic separation exhibits an inverse isotope effect just as has b een observed for gas chromatographic separations. An inverse isotope e ffect was observed for isotopomer pairs even when they were not separa ted sufficiently to exhibit two distinct peaks.