AMINO-ACID SEPARATION BY PREPARATIVE TEMPERATURE-SWING CHROMATOGRAPHYWITH FLOW REVERSAL

A special type of adsorptive parametric pumping called temperature-swi ng chromatography is experimentally investigated for a new application : the separation of amino acids. Experiments have been done with a mix ture of arginine, histidine and lysine in acidic solution (HCl). A mul ticomponent equilibrium model has been used to simulate the experiment al results and to investigate some possible ways to improve the separa tion. It is shown that it is possible to separate these amino acids, a nd the composition of the products strongly depends on the experimenta l conditions. Arginine with purity greater than 95% can be obtained by applying these methods.