SIZE-EXCLUSION CHROMATOGRAPHY ON SOFT PACKING MATERIAL UNDER AXIAL-COMPRESSION

The influence of soft gel axial compression on the packing structure a nd the associated chromatographic parameters such as dispersion of chr omatographic zones, retention volume and peak resolution have been con sidered for size-exclusion chromatography. Continuous time gel compres sion is accomplished by the use of a special column with controlled ex ternal pressure applied to the packing. The experimentally obtained im provement in resolution combined with the decrease in separation time has been proved.