DYNAMIC MODELING OF ELECTROPHORETICALLY MEDIATED MICROANALYSIS

A dynamic model is presented for simulation of reaction-based chemical analysis of enzymes and substrates in capillary electrophoretic syste ms by the methodology of electrophoretically mediated microanalysis (E MMA). The mathematical model utilizes mass balance expressions describ ing the time-dependent effects of electromigration, chemical reaction, and diffusional band broadening upon the concentration profiles of th e various reagent and product species. The model is implemented in an iterative computer program in which the capillary is segmented into ar rays of bins storing the concentration profiles of each of the chemica l species. During each time increment, the effects of electrophoresis, reaction kinetics, and diffusion are calculated, and the concentratio ns stored in the arrays are updated. The flexibility of the model to a ccommodate various initial capillary conditions, sample introduction m ethods, and voltage programming allows diverse EMMA analyses to be sim ulated. The simulated results are shown to be in good qualitative agre ement with experimental data for zonal injection and moving boundary E MMA determinations of leucine aminopeptidase as well as an EMMA analys is of ethanol.