COMPARATIVE CAPILLARY ELECTROPHORETIC AND NUCLEAR-MAGNETIC-RESONANCE STUDIES OF THE CHIRAL RECOGNITION OF RACEMIC METOMIDATE WITH CYCLODEXTRIN HOSTS

Detailed capillary electrophoretic (CE) and H-1 NMR studies of the chi ral recognition of racemic metomidate with various cyclodextrin hosts show that H-1 NMR spectrometry is a useful technique for the investiga tion of the chiral recognition mechanism in CE. NMR spectrometry provi des data about the inherent chiral recognition abilities of a given ch iral selector and allows to choose the optimal conditions for enantios eparations by CE. The effects of the type of the cyclodextrin host, th e pH and the ionic strength of the separation medium on the chiral rec ognition were studied with both techniques. Enantioselectivities obser ved in CE can be explained on the basis of self-mobilities of the chir al selectors and apparent binding constants which were calculated usin g H-1 NMR experiments.