DETERMINATION OF ACETYLCHOLINE BY ONLINE MICRODIALYSIS COUPLED WITH PRE-MICROBORE AND POST-MICROBORE COLUMN ENZYME REACTORS WITH ELECTROCHEMICAL DETECTION
Tr. Tsai et al., DETERMINATION OF ACETYLCHOLINE BY ONLINE MICRODIALYSIS COUPLED WITH PRE-MICROBORE AND POST-MICROBORE COLUMN ENZYME REACTORS WITH ELECTROCHEMICAL DETECTION, Journal of chromatography B. Biomedical applications, 678(2), 1996, pp. 151-155
Citations number
15
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical applications
A sensitive procedure consisting of a pre- and post-microbore column r
eactor sequence of a LC-electrochemical detection system coupled with
on-line microdialysis system is described in the present study to meas
ure endogenous acetylcholine concentration in freely moving rats. The
pre-column packed, with immobilized choline oxidase and catalase, was
used to remove choline, whereas the post-column, packed with immobiliz
ed acetylcholine oxidase and choline oxidase, was used to measure acet
ylcholine selectively, The detection limit of acetylcholine was found
to be 5 fmol/mu l (50 fmol/10 mu l). The usefulness of the described m
ethodology was evaluated by examining the change in the striatal acety
lcholine concentration of freely moving rats after physostigmine (0.5
mg/kg, s.c.) administration.