DETERMINATION OF A HIV PROTEASE INHIBITOR (DMP-450) IN ANIMAL AND HUMAN PLASMA BY SOLID-PHASE EXTRACTION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Extraction of DMP 450 from plasma was performed with C-2 solid-phase e xtraction columns, using 0.1 M ammonium acetate in 90% methanol to elu te DMP 450. The extraction recovery over the range of 10 to 10 000 ng/ ml averaged 81.0, 96.2, 77.4, 95.2 and 68.0% from rat, dog, monkey, ch impanzee (25-10 000 ng/ml) and human plasma, respectively. HPLC analys is was carried out with a C-18 column and a mobile phase of acetonitri le, methanol and 30 mM potassium phosphate (pH 3), the composition dep endent on the type of plasma being analyzed, and monitored at a wavele ngth of 229 nm. Intra-day and inter-day coefficients of variation were less than 9.9 and 12.9%, respectively. Absolute differences were less than 11.5%.