K. Lemr, HOMOLOG SEPARATION OF LINEAR ALCOHOL ETHOXYLATES BY HIGHPERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography, 732(2), 1996, pp. 299-305
Citations number
14
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
The optimization of a mobile phase and temperature for RP-HPLC separat
ion of 3,5-dinitrobenzoyl derivatives of linear alcohol ethoxylates (L
AEs) according to the alkyl chain length is reported. Partial inversio
n of the elution order of ethoxymers with a change of acetonitrile con
centration is employed. A decrease of the temperature allows a further
improvement in homologue separation. The addition of sodium perchlora
te reduces the retention of higher ethoxymers. This is used for final
separation improvement. The optimal conditions found were: stationary
phase: LiChrospher 100 RP-18 endcapped, 5 mu m; column: LiChroCart 250
x 4 mm I.D.; mobile phase: acetonitrile-water (98:2, v/v) with 0.0011
mol/l sodium perchlorate; flow-rate: 1.5 ml/min; temperature: 5 degre
es C; detection: UV absorption at 233 nm. These conditions were succes
sfully applied to the separation of a mixture of C-12-C-18 LAEs and no
nylphenol ethoxylates.