IMPROVED RAPID METHOD FOR THE ISOLATION, PURIFICATION AND IDENTIFICATION OF COLLAGEN GLYCOSIDES

An updated method for obtaining purified glucosylgalactosyl hydroxylys ine (Glc-Gal-Hyl) is presented. Marine sponge is subjected to alkaline hydrolysis followed by ion-exchange chromatography and gel filtration . Identification is confirmed by consecutive mild acid hydrolysis of t he extract to produce galactosyl hydroxylysine (Gal-Hyl) and hydroxyly sine (Hyl). High-performance liquid chromatography (HPLC) with a sodiu m acetate-acetonitrile buffer and dabsyl chloride as the derivatizing agent demonstrated the purity of the extract and its hydrolyzates. Glc -Gal-Hyl is not commercially available but this easy and efficient met hod can provide large amounts of it for collagen metabolism studies.