H. Garza et al., IMPROVED RAPID METHOD FOR THE ISOLATION, PURIFICATION AND IDENTIFICATION OF COLLAGEN GLYCOSIDES, Journal of chromatography, 732(2), 1996, pp. 385-389
Citations number
10
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
An updated method for obtaining purified glucosylgalactosyl hydroxylys
ine (Glc-Gal-Hyl) is presented. Marine sponge is subjected to alkaline
hydrolysis followed by ion-exchange chromatography and gel filtration
. Identification is confirmed by consecutive mild acid hydrolysis of t
he extract to produce galactosyl hydroxylysine (Gal-Hyl) and hydroxyly
sine (Hyl). High-performance liquid chromatography (HPLC) with a sodiu
m acetate-acetonitrile buffer and dabsyl chloride as the derivatizing
agent demonstrated the purity of the extract and its hydrolyzates. Glc
-Gal-Hyl is not commercially available but this easy and efficient met
hod can provide large amounts of it for collagen metabolism studies.