BIOPHYSICAL METHODS FOR THE DETERMINATION OF ANTIBODY-ANTIGEN AFFINITIES

Binding specificities against virtually any antigen can be isolated fr om antibody splayed on filamentous phage. The determination of antibod y-antigen affinity constants and binding kinetics is an important part of antibody characterization, and may be predictive of antibody perfo rmance in biotechnological applications. This article, intended as a g uideline for the scientist who isolates a novel antibody and wishes to characterize its binding properties, presents the authors' view on wi dely used methodologies for the quantitative determination of antibody -antigen interactions.