Hj. Spero et Dw. Lea, EXPERIMENTAL-DETERMINATION OF STABLE-ISOTOPE VARIABILITY IN GLOBIGERINA BULLOIDES - IMPLICATIONS FOR PALEOCEANOGRAPHIC RECONSTRUCTIONS, Marine micropaleontology, 28(3-4), 1996, pp. 231-246
We have quantified the environmental and physiological parameters resp
onsible for stable isotopic disequilibrium in the non-symbiotic plankt
ic foraminifera, Globigerina bulloides, via controlled experiments wit
h living specimens. Individual test chambers secreted in the laborator
y were amputated, pooled with other chambers from defined positions in
the shell whorl and analyzed for their carbon and oxygen isotopic com
position. When temperature, delta(18)O(water) and delta(13)C of Sigma
CO2 are kept constant, the chamber delta(13)C and delta(18)O values in
crease 2.6 and 0.8 parts per thousand respectively between the smalles
t chambers (chs. 1-9, shell size approximate to 180 mu m) and final ch
amber (ch. 14, shell size approximate to 500 mu m) Feeding experiments
with prey of different delta(13)C values show that 8-15% of the chamb
er delta(13)C signal is due to the incorporation of metabolic CO2. The
observed ontogenetic trend is responsible for the stable isotope size
-dependency in this species and may be due to a fractionation mechanis
m involving the incorporation of metabolic CO2 during calcification. T
emperature experiments show that shell delta(18)O varies as predicted
by paleotemperature equations, but is offset from equilibrium. We pres
ent correction factors that should be applied to delta(13)C and delta(
18)O data from well constrained size ranges to yield either oxygen iso
tope equilibrium or ambient delta(13)C of seawater Sigma CO2. Our resu
lts suggest that for paleoceanographic applications, shells in the 270
-320 mu m size range are optimal for paleoenvironmental reconstruction
s.