BETA-2 INTEGRINS AND ICAM-1 ARE INVOLVED IN ESTABLISHMENT OF THE INTESTINAL MUCOSAL T-CELL COMPARTMENT

Development of the mucosal immune system was examined in mice with par tial loss of expression of ICAM-1 or CD18. Profound effects on Peyer's patch (PP), lamina propria (LP), and intraepithelial lymphocyte (IEL) T cell populations were observed in mutant mice. Normal expression of CD18 integrins and ICAM-1 was essential for development of the CD8 al pha beta TCR alpha beta LP and IEL compartment and for the generation of normal PP lymphocyte populations. The partial loss of CD8 alpha bet a IEL correlated with the loss of TCR alpha beta IEL-mediated lytic ac tivity. The presence of a subset of Thy1(+) TCR gamma delta IEL was al so dependent on CD18 integrins and ICAM-1. Both the lytic activity and the expression of CD11c by TCR gamma delta IEL were up-regulated in t he presence of TCR alpha beta T cells. Analysis of bone marrow chimera s demonstrated that a bone marrow-derived ICAM-1(+) accessory cell was involved in the generation of some TCR alpha beta IEL. These results demonstrated that ICAM-1 and beta 2 integrins were required for establ ishment of a normal intestinal immune system.