A MOLECULARLY IMPRINTED NICOTINE-SELECTIVE POLYMER

A (-)-nicotine-selective polymer was prepared by molecular imprinting technique using methacrylic acid as a functional monomer. Liquid-chrom atographic tests, using the polymers as a stationary phase, exhibited that the basicity of the functional group of (-)-nicotine is crucial f or rebinding by the molecularly imprinted polymer. Scatchard analysis implied that tile binding sites generated within the polymer are heter ogeneous in terms of affinity, and the apparent dissociation constant of the highest affinity binding sites was estimated as 3.7 mu M.