ANALYSIS OF 76 KB OF THE CHLORELLA VIRUS PBCV-1 330-KB GENOME - MAP POSITION-182 TO POSITION-258

Analysis of 76 kb of newly sequenced DNA, located between map position s 182 and 258 kb in the 330-kb chlorella virus PBCV-1 genome, revealed 175 open reading frames (ORFs) of 65 codons or longer. One hundred an d five of these 175 ORFs were considered major ORFs. Twenty-one of the 105 major ORFs resembled proteins in databases including ribonucleoti de reductase small subunit, RNase III, thioredoxin, glutaredoxin, prot ein disulfide isomerase, deoxynucleoside kinase, frog virus 3 ATPase, Acetobacter cellulose synthase, a bacteriophage encoded endonuclease, and two C-5 cytosine DNA methyltransferases. One of the ORFs was the P BCV-1 major capsid protein. The 105 major ORFs were evenly distributed along the genome. One set of ORFs was separated by 543 nucleotides wh ereas 75 of the ORFs were separated by fewer than 100 nucleotides. Nin eteen of the 175 ORFs resembled other PBCV-1 ORFs, suggesting that the y represent either gene duplications or gene families. (C) 1996 Academ ic Press, Inc.