THE EFFECT OF 5-HT AND SELECTIVE 5-HT RECEPTOR AGONISTS AND ANTAGONISTS ON RAT DORSAL VAGAL PREGANGLIONIC NEURONS IN-VITRO

1 Whole-cell patch-clamp recordings were made from 142 visually identi fied rat dorsal vagal preganglionic neurones (DVMs). Applications of 5 -hydroxytryptamine (5-HT, 20 mu M, 2 min) elicited a slow depolarizati on (8.2 +/- 0.5 mV, n = 59) in 95% of the cells tested, accompanied by an increase in excitability. In (68%) of DVMs the depolarization was associated with an increase in apparent membrane resistance (R(m), 22. 7 +/- 2.2%). These depolarizations and increases in R(m) (14.3 +/- 2.6 %, n = 8) were maintained in a medium which blocked synaptic transmiss ion. 2 The response to 5-HT was associated with a reversal potential ( E(rev)) of -91 +/- 1 mV at an extracellular K+ concentration ([K+](o)) of 4.2 mM. This correlated well with the K+ equilibrium potential (E( K) = -89 mV). 3 The depolarizing effect of 5-HT was attenuated by the 5-HT2A/2C receptor antagonists, ketanserin (1 mu M), LY 53,857 (1 mu M ) and the 5-HT1A/2A receptor antagonist, spiperone (1 mu M). The 5-HT1 A receptor antagonist, pindobind 5-HT1A (5 mu M), had no effect on the depolarizing response to 5-HT. 4 The effect of 5-HT was mimicked by t he 5-HT2A/2C receptor agonist, alpha-methyl-5-HT (50 mu M), the 5-HT1 receptor agonist, 5-carboxamidotryptamine (20 mu M) and the putative 5 -HT4 agonist, 5-methyoxytryptamine (50 mu M). The selective 5-HT4 rece ptor antagonist, GR113808, had no effect on the depolarizing effect of 5-HT or 5-MEOT on DVMs. 5 The 5-HT3 antagonists, MDL 72222 (10 mu M) and ICS-205-930 (1 and 10 mu M), partially reduced the effect of 5-HT. The 5-HT3 receptor agonist, 2-methyl-5-HT (100-300 mu M), excited a p roportion of neurones tested (56%) by evoking a depolarizing and/or an increase in postsynaptic potentials (p.s.ps). 6 These results are con sistent with direct, postsynaptic actions of 5-HT on DVMs via 5-HT2A r eceptors, being mediated, in part, by the reduction of K+ conductance.