Cn. Penfold et al., CHARACTERIZATION OF GENES INVOLVED IN BIOSYNTHESIS OF CORONAFACIC ACID, THE POLYKETIDE COMPONENT OF THE PHYTOTOXIN CORONATINE, Gene, 183(1-2), 1996, pp. 167-173
Coronafacic acid (CFA) is the polyketide component of coronatine (COR)
, a phytotoxin produced by the plant pathogen, Pseudomonas syringae. I
n the present study we have determined the nucleotide sequence of a 3.
92-kb DNA fragment involved in CFA biosynthesis. Analysis of the seque
nce revealed four complete open reading frames (ORFs) designated cfa1
to cfa4 and one incomplete ORF (cfa5), all transcribed in the same dir
ection. The predicted translation products of cfa1, cfa2 and cfa3 show
ed relatedness to acyl carrier proteins, fatty acid dehydrases and bet
a-ketoacylsynthases, respectively, which are required for polyketide s
ynthesis. cfa1 was subcloned, its sequence was confirmed, and it was o
verexpressed in E. coli to yield a peptide with an apparent molecular
mass of 6 kDa.