THE EXPRESSION OF ESTROGEN-RECEPTOR AND ESTROGEN EFFECT IN MBA-15 MARROW STROMAL OSTEOBLASTS

MBA-15, a marrow stromal-derived cell line, was shown to express an es trogen receptor. This finding was confirmed by in situ hybridization a nd receptor binding assay. An exposure to estrogen (10(-12)-10(-6) M) in a dose response manner resulted in a decrease of cell proliferation as measured by MTT assay. Cell function was measured by enzymatic act ivities of two osteoblastic markers, CD10/NEP and alkaline phosphatase . These enzymatic activities were elevated following the estrogen trea tment. This model enabled direct evaluation of the estrogen effect on stromal osteoblast cells. (C) 1996 Academic Press Limited