A SINGLE VECTOR CLONING, MUTAGENESIS AND EXPRESSION SYSTEM

Authors
TOWLER EM STEBBINS JW DEBOUCK C
Citation
Em. Towler et al., A SINGLE VECTOR CLONING, MUTAGENESIS AND EXPRESSION SYSTEM, Gene, 183(1-2), 1996, pp. 259-263
Citations number
20
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
183
Issue
1-2
Year of publication
1996
Pages
259 - 263
Database
ISI
SICI code
0378-1119(1996)183:1-2<259:ASVCMA>2.0.ZU;2-O
Abstract
We describe a multipurpose Escherichia coli vector, pOTSflblue, that c an be utilized for high efficiency subcloning, epitope-tagged protein overexpression, authentic protein overexpression and efficient mutagen esis.